Job Description

Job Description

Job Description

Salary: $80k - $85K

About Us

Monod Bio is a pioneer company in the design ofde novoprotein reagents for Life Sciences and Diagnostics. Our innovative design platform technology takes advantage of the most recent advances in deep learning computational protein engineering to create highly rapid and sensitive protein-based reagents and assays.Our visionary founding team and state-of-the-art technology and product development experience position Monod Bio at the forefront of molecular reporter, binder and functional protein innovation, and we welcome exceptional individuals to join us in this journey.

Position Summary

Monod Bio is seeking a Temporary Senior Research Associate in Protein Manufacturing and Molecular Biology to support our protein manufacturing team. In this role, you will contribute to the screening, identification, and production of computationally designed de novo proteins. The ideal candidate will have hands-on experience in molecular biology, protein expression in E. coli , purification, and biochemical analysis techniques such as chromatography and spectroscopy.

Key Responsibilities

• Perform molecular biology and protein manufacturing tasks assigned by the team lead.
• Assist in experimental planning and troubleshooting under the guidance of senior scientists.
• Maintain accurate records of plasmids and purified protein lots in Benchling.
• Assemble DNA constructs from synthetic gene fragments using Golden Gate cloning.
• Execute standard molecular biology techniques including PCR, cloning, plasmid prep, and DNA purification.
• Conduct high-throughput protein expression screening in E. coli using 96-well plate formats.
• Perform binding assays using Bio-Layer Interferometry (BLI).
• Purify proteins using affinity chromatography (Ni-NTA, Strep-Tactin) and size exclusion chromatography (SEC).
• Characterize protein quality and stability using SEC-MALS, circular dichroism, and thermostability assays.
• Collaborate with the team to fulfill protein production requests and ensure timely delivery.
• Support additional lab operations and projects as needed.

Required Qualifications

• A bachelors degree in biochemistry, biophysics, analytical chemistry, biology, biotechnology, chemical engineering, or related fields.
• 2+ years of experience in protein sciences, purification, and analysis in academia or industry.
• Experience with molecular biology techniques (DNA work, PCR, cloning and plasmid purification).
• Proficiency with coli bacterial protein expression and purification using chromatography.
• Familiarity with biochemical and biophysical methods such as BLI, FPLC, SDS-PAGE, and SEC-MALS.
• Extensive experience with tracking data and recording results in electronic lab notebook format (e.g., Benchling).
• Highly organized and detail-oriented, with strong communication skills and the ability to work independently.

Preferred Qualification

• Experience designing expression vectors for both prokaryotic and eukaryotic systems.

Physical Environment & Key Requirements

• Ability to perform work in both an office and laboratory environment. Will be in proximity to BSL2 labs. Familiarity with biological and chemical hazard handling. Training and equipment will be provided, as necessary.
• Office mobility and the ability to stand for periods of time as needed is required.
• Operations happen in moderately loud environments. PPE included by the company.
• Mild odors from the manufacture of physical specimens and from laboratory work. The environment has been tested, and proper engineering protections are in place.

What We Offer

• Salary range of $80K to $85K
• State-of-the-art lab and office on a bus line and near coffee shops, restaurants, and South Lake Union neighborhood.
• Casual environment, friendly colleagues, and of course, excellent snacks in the kitchen!

Every position requires certain physical capabilities with the major ones for this role noted above. Monod Bio seeks to provide reasonable accommodations that enable individuals with disabilities to perform essential duties when possible. Equal employment opportunity is a fundamental principle at Monod Bio. We are committed to nondiscrimination in all business operations. We are proud to enjoy a respectful and inclusive work environment where everyone has the opportunity to thrive.

Job Tags

Similar Jobs